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Academic Staff |
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Contact Details |
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Department of Cancer Biology (Research)
Direct :
079-22688000 ; 079-22690000
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Services :

The department is recently equipped with Metafer, automatic metaphase and interphase scanning and
capturing system for Karyotyping and FISH (Fluorescence In Situ Hybridization) assays. It has fully motorized epi-fluorescence microscope (Imager.Z2) from Zeiss, Germany with special filter sets for FISH and multicolor FISH. It
has IKAROS software for Karyotyping and ISIS software for FISH by Metasystems, Germany. The system also has
facility of automatic spot counting for FISH analysis for hematological malignancies and solid tumor using
Metacyte software. It has 4 review stations. Moreover, the division also has three other systems from
Metasystems, Germany and Cytovision software by Applied Imaging, USA. In addition to Karyotyping, FISH
(Fluorescence in situ hybridization), M-FISH (multicolor FISH) and generation of custom made FISH probes from
Bacterial artificial chromosomes also provides precise chromosomal breakpoint mapping in leukemia as well as
in solid tumor patients. For FISH automation, hybridization and, denaturation chamber (Thermobrite, Vysis,
USA) helps in rapid and consistent FISH assays. The facility for multiplex-FISH is first of its kind in the country.
Cell Biology Division is equipped with the state of art set up for research in the area of clinical cytogenetics. The
division is actively engaged in research on various aspects of chromosomal abnormalities. Major clinical services
provided by the division are for diagnosis and prognostication of patients with various hematological
malignancies. The services are provided to the GCRI patients as well as other cases referred from various other
places.
Clinical Activities :
The division caters extensive clinical cytogenetic
services for leukemia patients. It includes karyotype and
FISH analysis in CML, AML, and various other
hematological malignancies to help diagnosis and
prognosis. The department has also initiated solid
tumor FISH from FFPE sections. It includes HER-2 neu
gene rearrangements in breast cancer, N-MYC gene
rearrangements in neuroblastoma, i(12p) gene
rearrangements in Germ Cell Tumor and ALK gene
rearrangements in NSCLC. Academic Activities :
The division enrolled graduate and post-graduate
students from universities across India for observership, project work and dissertation. Staff of the
division is involved in M.Sc. Cancer Biology course for
theory and practical work, initiated by Department of
Cancer Biology at GCRI.
Research Activities :
The main research thrust of the division is chromosome
analysis of leukemia patients. Chromosomal anomalies
are documented at diagnosis and during later stages of
the disease, which aids in prognostication and clinical
research. Metaphase chromosomes are obtained
following short-term cultures of peripheral blood
lymphocytes and bone marrow. The novel chromosomal
anomalies as per the leukemia subtypes are contributed
to existing pool of databases of chromosomal
aberrations in leukemia by publishing in national and
international journals. Karyotype analysis by GTG
banded metaphases is substantiated by locus specific
and/or whole chromosome FISH in leukemia and solid
tumor, and multiplex-FISH for increased sensitivity of
chromosome anomaly detection. Generation of custom
made FISH probes from Bacterial artifficial
chromosomes to identify novel chromosomal
breakpoints.
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